Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii.

نویسندگان

  • N R Mullane
  • M Ryan
  • C Iversen
  • M Murphy
  • P O'Gaora
  • T Quinn
  • P Whyte
  • P G Wall
  • S Fanning
چکیده

The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled with multiple fluorescent dyes. This approach was applied to a collection of 112 isolates representing all 16 of the currently defined E. sakazakii biogroups. MLVA successfully discriminated among these isolates and compared favorably with pulsed-field gel electrophoresis. The method was relatively fast and easy to perform. The potential value of MLVA as an epidemiological tool is discussed.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 74 4  شماره 

صفحات  -

تاریخ انتشار 2008